Characterization of Simple Sequence Repeats from Human ESTs and Creation of a Comprehensive Data Base
by Amit kumar
Abstract: The aim of the present analysis is to create a complete database of Simple Sequence Repeats (SSRs) occurring in the Expressed Sequence Tags (ESTs) of Human Genome. For the recognition of SSRs, various Bioinformatics Tools, Databases and Softwares are employed. The data are analyzed statistically for Mean, Density and Frequency distribution of the identified Repeats. A consolidated Database is created and maintained in SAS Environment. This database can provide comprehensive information regarding (1) the complete sequence of Microsatellites/Tandem repeats, (2) the number of times each one is repeated, (3) the starting and ending position of each repeat, and (4) its length. In addition the database can also provide the location of the gene and also its function. As these Microsatellites are known to be widely used in a variety of fundamental and applied fields of life and medical sciences, the availability and accessibility to such comprehensive data base would greatly help the researchers.
Keywords: Biomarkers, EST, Expressed sequence tags, Statistical analysis, Single sequence repeats, DNA microarrays, differential expression analysis, biological database, SAS, microsatellites, bioinformatics tools, Human genome
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